Twenty-year-olds Karl Johnson, Travis Burns and Matt Ballin have been given the opportunity to impress before Manly's premiership opener against NZ Warriors on March 13.
Parramatta recruit Michael Witt will start at halfback while recruits Terry Hill, Ben Kennedy and Brent Kite will come off the bench.
The trial will be held in quarters.
Manly: Brett Stewart, Scott Donald, Paul Stephenson, Karl Johnson, John Hopoate, Michael Witt, Travis Burns, Kylie Leuluai, Matt Ballin, Daniel Heckenberg, Sam Harris, Glenn Stewart, Luke Williamson. Interchange: Terry Hill, Chris Hicks, Michael Monaghan, Jason King, Chad Randall, Shane Dunley, Brent Kite, Ben Kennedy, Steve Menzies.